MegaFi™ Pro Fidelity DNA Polymerase

Cat. No.
G886
Unit
400 rxn
Price
$165.00

PCR buffet

Cat. No. G886
Name MegaFi™ Pro Fidelity DNA Polymerase
Unit 400 rxn
Category PCR Polymerase
Description

MegaFi™ Pro Fidelity DNA Polymerase sets a new benchmark for specific, sensitive, robust, and high-fidelity PCR performance. This advanced, strategically engineered enzyme is combined with a monoclonal antibody that inhibits both 5’-3’ polymerase activity and 3'-5’ exonuclease activity at room temperature, enabling precise Hot Start reactions. With exceptional sensitivity, MegaFi™ Pro can amplify even the most challenging templates, outperforming leading competitors. It delivers unparalleled high fidelity with an ultra-low error rate—over 2,000 times lower than Taq polymerase—making it the most accurate enzyme available. Ideal for demanding applications such as Next Generation Sequencing, molecular cloning, and diagnostics, MegaFi™ Pro ensures the highest quality results for your PCR needs.

Product Features:

  • 2000X lower error rates than regular Taq
  • Amplifies difficult templates
  • Blunt ends
  • Also available as a PCR MasterMix (Cat. No. G887)
  • Included in abm's PCR Buffet Program

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Product Component Quantity
MegaFi™ Pro Fidelity DNA Polymerase 400 rxn (200 µl)
2X MegaFi™ Pro Buffer1 5.0 ml (4 x 1.25 ml)

1 Buffer contains 2 mm Mg2+

ISO 13485:2016 MDSAP Certified

ISO 13485:2016 MDSAP Certified
Our PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).

Application
  • Ultra high-fidelity PCR 
  • Next Generation Sequencing
  • Accurate CRISPR Edit Detection
  • Mutagenesis
Storage Condition

Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly.

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G886
  • Batel, A., Polović, M., Glumac, M. et al. SPRTN is involved in hepatocellular carcinoma development through the ER stress response. Cancer Gene Ther 31, 376–386 (2024). https://doi.org/10.1038/s41417-023-00708-w

    Chaudhuri, E., Jang, S., Chakraborty, R., Radhakrishnan, R., Arnarson, B., Prakash, P., ... & Dash, C. (2025). CPSF6 Promotes HIV-1 Preintegration Complex Function. bioRxiv, 2025-01. https://doi.org/10.1101/2025.01.28.635394

    Dou, C., Hu, L., Ding, X., Chen, F., Li, X., Wei, G., & Yan, Z. (2024). Microbiota Alterations in Lung, Ileum, and Colon of Guinea Pigs with Cough Variant Asthma. International Journal of Molecular Sciences, 25(4), 2449. https://doi.org/10.3390/ijms25042449

    Padron, A., Dwivedi, R., Chakraborty, R., Prakash, P., Kim, K., Shi, J., ... & Dash, C. (2024). Cyclophilin A facilitates HIV-1 integration. Journal of Virology, 98(11), e00947-24. https://doi.org/10.1128/jvi.00947-24

    Xian, J., Chen, Q., Zhang, C., Huang, Q., Zhang, L., Ma, J., ... & Zhang, J. (2023). Polygonati rhizoma polysaccharides relieve exercise-induced fatigue by regulating gut microbiota. Journal of Functional Foods, 107, 105658. https://doi.org/10.1016/j.jff.2023.105658

    Zhang, X., Bi, L., Gentekaki, E., Zhao, J., Shen, P., & Zhang, Q. (2023). Culture-independent single-cell PacBio sequencing reveals epibiotic Variovorax and nucleus associated Mycoplasma in the microbiome of the marine benthic protist Geleia sp. YT (Ciliophora, Karyorelictea). Microorganisms, 11(6), 1500. https://doi.org/10.3390/microorganisms11061500

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