Protease Inhibition Cocktail with EDTA

Cat. No.
G135E
Unit
1.0 ml
Price
$178.00
Cat. No. G135E
Name Protease Inhibition Cocktail with EDTA
Unit 1.0 ml
Category Reagents and Chemicals
Description

Protease Inhibitor Cocktail is used to prevent the digestion of proteins that occurs after cell lysis takes place. The presence of EDTA ensures greater inhibition of metalloproteases.

Recommended Protocol 1mL of the Protesase Inhibitor Cocktail is recommended for the inhibition of protease activity form 100mL of cell lysate at a density of 10^8 cells per mL.

However, since not all organisms contain the same level of endogenous proteases, it is up to the researcher to optimize the concentration for their experiments.

Storage Condition

Store cocktail at -20°C. Cocktail is dissolved in DMSO containing little deionized water. The cocktail may form crystals. If there are crystals, place it at RT for 5 minutes. The cocktail is stable for a year.

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G135E
Print/Download Datasheet
  • Kim, S., Lee, M. S., Jung, S., Son, H. Y., Park, S., Kang, B., ... & Kim, Y. "Ginger Extract Ameliorates Obesity and Inflammation via Regulating MicroRNA-21/132 Expression and AMPK Activation in White Adipose Tissue"  Nutrients  10(11):1567 (2018).
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