Recombinant Human CCL21 (E. coli)

Cat. No.
Z100195
Unit
Price
$92.00
Cat. No. Z100195
Name Recombinant Human CCL21 (E. coli)
Description CCL21, which is a member of the family of CC-Chemokines, has a unique 36 (murine) or 37 (human) amino acid carboxyl-terminal extension not seen in other chemokines. CCL21 selectively stimulates the chemotaxis of adult T lymphocytes and is expressed preferentially in lymph node tissue. CCL21 is critical for adult T cell adhesion to high endothelial venules in lymph nodes, a rate-limiting step for T cell trafficking through nodal tissue.
Organism Human (H. sapiens)
Unit quantity 20 µg
Family Chemokine-beta Family (CC)
Aliases 6Ckine, CKb9, ECL, SCYA21, SLC, TCA4
Gene Symbol CCL21
Gene ID 6366
Accession Number O00585
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 12 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity >95% as determined by SDS-PAGE
Formulation Recombinant CCL21 was lyophilized from a 0.2 μm filtered solution in 2.5% glycine, 0.5% sucrose, 0.01% Tween80, 5 mM Glutamic acid, pH 4.5.
Function Determined by its ability to chemoattract total lymphocyte population using a concentration range of 10-100 ng/mL.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100195
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