Recombinant Human Chemerin (E. coli)

Cat. No.
Z102205
Unit
Price
$290.00
Cat. No. Z102205
Name Recombinant Human Chemerin (E. coli)
Description Human Chemerin, also known as Tazarotene induced Gene 2 (TIG2), is an adipokine that regulates adipogenesis, metabolism and inflammation through activation of the chemokine-like receptor 1. Its other ligands include G protein-coupled receptor 1 and chemokine receptor-like 2. Chemerin is expressed in various tissues where its biologically active form is generated by proteolytic removal of C-terminal residues by circulating proteases. In human, the soluble protoform gets processed into a monomeric, heparin binding bioactive molecule that can have both pro- and anti-inflammatory properties depending on the modality of enzymatic cleavage by different classes of proteases.
Organism Human (H. sapiens)
Unit quantity 25 µg
Family Other Growth Factors/Cytokines
Aliases HP10433, TIG2
Gene Symbol RARRES2
Gene ID 5919
Accession Number Q99969
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 16 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2µm filtered solution containing 0.1% TFA
Function Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human ChemR23. The ED(50) for this effect is typically 4 - 20 ng/mL.
Reconstitution A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z102205
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