Recombinant Human HGF

Cat. No.
Z102785
Unit
Price
$200.00
Cat. No. Z102785
Name Recombinant Human HGF
Description

Hepatocyte growth factor (HGF) is a multifunctional cytokine that was originally described as a mesenchymal-derived factor that regulates cell growth, cell motility, morphogenesis and angiogenesis through activation of its receptor, the transmembrane tyrosine kinase encoded by the c-Met proto-oncogene. HGF and c-Met are often co-expressed or over-expressed in a variety of human malignancies including glioblastoma and medullablastoma. The multifunctional effects of HGF: c-Met signaling in tumor cells are mediated by a network of signal transduction pathways including mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K). HGF consists of two subunits held by a disulfide bond. The alpha subunit (69 kDa) has a length of 440 amino acids and the beta subunit (34 kDa) has a length of 234 amino acids. The beta chain of the factor shows approximately 38% homology at the protein level to the serine protease domain of plasminogen. Recombinant HGF expressed in human 293 cells and has a molecular mass of 70 kDa, lower compared to the Molecular mass for this cytokine expressed in CHO cells which emphasizes the difference in post translational modifications.

Organism Human (H. sapiens)
Unit quantity 5 µg
Family Other Growth Factors/Cytokines
Aliases

DFNB39, F-TCF, HGFB, HPTA, SF

Gene Symbol HGF
Gene ID 3082
Accession Number P14210
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 79.4 kDa
Endotoxin Level <1 EU/µg
Purity

≥ 95% by SDS-PAGE gel and HPLC analyses

Function Determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells. The expected ED50 for this effect is 1.0-20.0 ng/ml.
Reconstitution A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration of 0.1-1 mg/ml. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z102785
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