Recombinant Human Midkine (NEGF2) (E. coli)

Cat. No.
Z102175
Unit
Price
$103.00
Cat. No. Z102175
Name Recombinant Human Midkine (NEGF2) (E. coli)
Description Midkine and its functionally-related protein Pleiotrophin (PTN), are heparin-binding neurotrophic factors that signal through the same receptor. Midkine is involved in a number of biological processes including the migration of neutrophils, inflammatory leukocytes, macrophages, neuronal cells, and osteoclasts. Midkine is a basic, non-glycosylated polypeptide that contains five intra-chain disulfide bonds. There is 87% identity between the human and murine Midkine and approximately 50% identity between human Midkine and human PTN with the conservation of all 10 cysteines.
Organism Human (H. sapiens)
Unit quantity 20 µg
Family VEGF/PGDF/Pleiotrophin Family
Aliases ARAP, MK, NEGF2
Gene Symbol MDK
Gene ID 4192
Accession Number P21741
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 14 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 µm filtered solution in PBS
Function The ED(50) was determined by the dose-dependent proliferation of AsPC-1 cells and was found to be <0.5ng/mL.
Reconstitution Reconstitute at 0.1 mg/ml in sterile PBS
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z102175
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