Recombinant Human TNF (E. coli)

Cat. No.
Z100855
Unit
Price
$92.00
Cat. No. Z100855
Name Recombinant Human TNF (E. coli)
Description Tumor Necrosis Factor is secreted by macrophages, monocytes, neutrophils, T cells, natural killer cells following their stimulation by bacterial lipopolysaccharides although cells expressing CD8(+) secrete little or no TNF-alpha. In addition, TNF is secreted by peripheral neutrophilic granulocytes and by a number of transformed cell lines that include those of astrocytes, microglial cells, smooth muscle cells and fibroblasts. Human milk also contains this factor. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, GM-CSF, SP, Bradykinin, Immune complexes, inhibitors of cyclooxygenase and platelet activating factor. TNF-alpha shares approximately 30% homology with TNF-beta. Human TNF-alpha is a non-glycosylated protein of 17.6 kDa.
Organism Human (H. sapiens)
Unit quantity 50 µg
Family TNF Super-family
Aliases DIF, IMD127, TNF-alpha, TNFA, TNFSF2, TNLG1F
Gene Symbol TNF
Gene ID 7124
Accession Number P01375
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 17.6 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 μm filtered solution in PBS (pH 7.2)
Function The ED50 as determined by dose dependent proliferation of IL4 stimulated human megakaryocytic cell line was found to be ≤0.5 ng/mL
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100855
Print/Download Datasheet
  • Tang, X., McMullen, T. P., & Brindley, D. N. "Increasing the low lipid phosphate phosphatase 1 activity in breast cancer cells decreases transcription by AP-1 and expressions of matrix metalloproteinases and cyclin D1/D3" Theranostics 9(21):6129 (2019).
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