Recombinant Human TNF

Cat. No.
Z101385
Unit
50 µg
Price
$760.00
Cat. No. Z101385
Name Recombinant Human TNF
Description Tumor Necrosis Factor is secreted by macrophages, monocytes, neutrophils, T cells, natural killer cells following their stimulation by bacterial lipopolysaccharides. Cells expressing CD4 secrete TNF-alpha while CD8(+) cells secrete little or no TNF-alpha. Stimulated peripheral neutrophilic granulocytes but also unstimulated cells and also a number of transformed cell lines, astrocytes, microglial cells, smooth muscle cells, and fibroblasts also secrete TNF. Human milk also contains this factor. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL-2, GM-CSF, SP, Bradykinin, immune complexes, inhibitors of cyclooxygenase, and PAF (platelet activating factor). Human TNF-alpha is a non-glycosylated protein of 17 kDa and a length of 157 amino acids. Murine TNF-alpha is N-glycosylated. Homology with TNF-beta is approximately 30%. TNF-alpha forms dimers and trimers.
Organism Human (H. sapiens)
Unit quantity 50 µg
Family TNF Super-family
Aliases DIF, IMD127, TNF-alpha, TNFA, TNFSF2, TNLG1F
Gene Symbol TNF
Gene ID 7124
Accession Number P01375
Source CHO cells
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 18 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity >95% as determined by SDS-PAGE
Formulation TNF-a was lyophilized from a 0.2μm filtered concentrated (1.0 mg/mL) solution in PBS, pH 7.2.
Function The ED(50) was determined cytolysis of murine L929 cells in the presence of Actinomycin D is ≤ 0.02 ng/mL, corresponding to a specific activity of > 5.0 x 10^7 units/mg.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z101385
Print/Download Datasheet
There are no references for this product yet!
This product has no review yet.
Controls and Related Product:

CAT.NO
Z100139
UNIT
1.0 mg
PRICE
$222.00

CAT.NO
Z100345
UNIT
100 µg
PRICE
$92.00

CAT.NO
Z100815
UNIT
10 µg
PRICE
$125.00

CAT.NO
Z100895
UNIT
10 µg
PRICE
$92.00

CAT.NO
Z101055
UNIT
10 µg
PRICE
$162.00

CAT.NO
Z101455
UNIT
50 µg
PRICE
$54.00