Recombinant Mouse GH1 (E. coli)

Cat. No.
Z200065
Unit
Price
$92.00
Cat. No. Z200065
Name Recombinant Mouse GH1 (E. coli)
Description The physiological activity for which growth hormone is best known is the promotion of growth of bone, cartilage, and soft tissues. Detectable levels of growth hormone are found throughout the remainder of adulthood, suggesting other functions in addition to promotion of growth. Growth hormone may be important for the maintenance of lean body mass; most growth-promoting effects of growth hormone are mediated by IGF-1 the synthesis of which is regulated by growth hormone. The biological activities of growth hormone are mediated by receptors belonging to one of the Cytokine receptor families. Growth hormone has been shown to be produced by T cells, B cells, and macrophages. In human lymphocytes growth hormone appears to up-regulate its own expression. Growth hormone appears to act as an enhancer of immune responses and is produced in considerable amounts by T helper cells.
Organism Mouse (M. musculus)
Unit quantity 50 µg
Family Somatotropin/Sclerostin/Serpin Families
Aliases Gh1, Ghb1
Gene Symbol GH1
Gene ID 14599
Accession Number P06880
Source E. coli
Species Mouse (M. musculus)
Appearance Lyophilized Powder
Molecular Weight 22 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from 0.2 μm filtered solution in Tris and NaCl
Function The ED50 was determined by the dose-dependent proliferation assay using the mouse myoblast cell line and was to be <0.5ng/ml.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200065
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