Recombinant Mouse IL6 (E. coli)

Cat. No.
Z200175
Unit
Price
$205.00
Cat. No. Z200175
Name Recombinant Mouse IL6 (E. coli)
Description IL6 is produced by many different cell types. The main sources in vivo are stimulated monocytes, fibroblasts, and endothelial cells. Macrophages, T cells and B-lymphocytes, granulocytes, smooth muscle cells, eosinophils, chondrocytes, osteoblasts, mast cells, glial cells, and keratinocytes also produce IL6 after stimulation. IL6 is a member of a family of cytokines, which also includes LIF, CNTF, Oncostatin M, IL11, and CT-1. All known members of the IL6 cytokine family induce hepatic expression of acute phase proteins. The IL6 receptor is expressed on T cells, mitogen-activated B cells, peripheral monocytes and some macrophage and B cell derived tumor cell types. It is not expressed in resting B cells but in resting T cells. The IL6 receptor, designated CD126, is a strongly glycosylated protein of 80 kDa. Recombinant Mouse IL6 is a 22 kDa polypepdtide with two intramolecular disulfide bridges.
Organism Mouse (M. musculus)
Unit quantity 10 µg
Family IL-6 Family
Aliases Il-6
Gene Symbol IL6
Gene ID 16193
Accession Number P08505
Source E. coli
Species Mouse (M. musculus)
Appearance Lyophilized Powder
Molecular Weight 22 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 μm filtered solution in PBS
Function The ED50 as determined by the dose-dependent proliferation of murine hybridoma was <0.05ng/ml.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200175
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