Recombinant Mouse MCP-5 (CCL12) (E. coli)

Cat. No.
Z200385
Unit
Price
$92.00
Cat. No. Z200385
Name Recombinant Mouse MCP-5 (CCL12) (E. coli)
Description MCP-5 (CCL12) is a chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. It is also a potent active monocyte chemokine that signals through CCR2. It is Involved in allergic inflammation and the host response to pathogens. Also, it may play a pivotal role during early stages of allergic lung inflammation.
Organism Mouse (M. musculus)
Unit quantity 25 µg
Family Chemokine-beta Family (CC)
Aliases MCP-5, Scya12
Gene Symbol CCL12
Gene ID 20293
Accession Number Q62401
Source E. coli
Species Mouse (M. musculus)
Appearance Lyophilized Powder
Molecular Weight 10 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity >95% as determined by SDS-PAGE
Formulation Recombinant MCP-5 was lyophilized from a 0.2 μm filtered 20 mM PB solution pH 7.5, 100 mM NaCl.
Function Determined by its ability to chemoattract human monocytes using a concentration range of 20-40 ng/mL.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200385
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