Recombinant Mouse MIP-3 (CCL19)

Cat. No.
Z200455
Unit
Price
$320.00
Cat. No. Z200455
Name Recombinant Mouse MIP-3 (CCL19)
Description The chemokine MIP-3 beta is a chemoattractant for T and B lymphocytes, dendritic cells, macrophage progenitor cells, and natural killer cells. It might, therefore, play an important role in the trafficking of T cells in the thymus and migration of T and B cells to secondary lymphoid organs. Furthermore, MIP-3 beta has been recently shown to mediate rapid adhesion of naive CD4(+) T lymphocytes to activated endothelial cells supporting the role of this chemokine in regulation of lymphocyte homing. MIP-3 beta acts through CC chemokine receptor 7 (CCR7). The chemokines Lkn-1 and MIP-3 alpha are also lymphocyte attractants, although compared with MIP- 3 beta, their promigratory effect on lymphocytes is less intensive and their chemotactic activity is more promiscuous. Lkn-1 attracts monocytes, lymphocytes, and eosinophils via the chemokine receptors CCR1 and CCR3. MIP-3 alpha exhibits promigratory effects on lymphocytes, neutrophils, and immature dendritic cells via the CCR6 receptor.
Organism Mouse (M. musculus)
Unit quantity 20 µg
Family Chemokine-beta Family (CC)
Aliases CKb11, ELC, Gm2023, MIP3B, Scya19, exodus-3
Gene Symbol CCL19
Gene ID 24047
Accession Number O70460
Source E. coli
Species Mouse (M. musculus)
Appearance Lyophilized Powder
Molecular Weight 10 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity >95% as determined by SDS-PAGE
Formulation Recombinant CCL19 was lyophilized from a 0.2 μm filtered 20 mM PB,100 mM NaCl solution pH 7.5.
Function Determined by its ability to chemoattract human dendritic cells using a concentration range of 10-50 ng/mL.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200455
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