Recombinant Mouse Noggin (NOG)

Cat. No.
Z200235
Unit
20 µg
Price
$320.00
Cat. No. Z200235
Name Recombinant Mouse Noggin (NOG)
Description Noggin is a secreted homodimeric glycoprotein that binds to ligands of the TGF-beta family (BMPs) and regulate their activity by inhibiting their access to signaling receptors. Mature human Noggin protein contains an N-terminal acidic region, a central basic heparin-binding segment and a C-terminal cysteine knot structure. Noggin binds different BMPs with variable affinities, antagonizing specific BMPs during skeletal development. Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin. Targeted deletion of Noggin in mice is known to result in severely malformed skeletal system and prenatal death while over-expression of Noggin in mature osteoblasts has been associated with severe osteoporosis.
Organism Mouse (M. musculus)
Unit quantity 20 µg
Family Other Growth Factors/Cytokines
Aliases DCYTB, FLJ23462, FRRS3
Gene Symbol NOG
Gene ID 18121
Accession Number P97466
Source E. coli
Species Mouse (M. musculus)
Appearance Lyophilized Powder
Molecular Weight 23 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 μm filtered solution in PBS (pH 7)
Function The ED50 as determined by induced alkaline phosphatase secretion in ATDC cells was determined to be ≤ 2.0 ng/mL
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200235
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