Scrambled siRNA GFP Lentivirus
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Product Name
Scrambled siRNA GFP Lentivirus -
Unit
4 x 500 μl -
Description
This ready-to-use lentivirus is part of abm’s iLenti™ RNAi Expression System and can be used to knockdown your gene of interest in tranduced target cells. A GFP reporter and puromycin selection marker allow for selection of successfully transduced target cells.
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Vector Map
piLenti-siRNA-GFP-Scrambled (click blue link to view) -
Titer
107 IU/mL -
Storage Condition
Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles.
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Disclaimer
Print/Download Datasheet
Koo, JW et al. "Epigenetic basis of opiate suppression of Bdnf gene expression in the ventral tegmental area" Nat. Neurosci. 8(3):415-422 (2015). DOI: 10.1038/nn.3932. PubMed: 25643298.
Korb, E et al. "BET protein Brd4 activates transcription in neurons and BET inhibitor Jq1 blocks memory in mice" Nat Neurosci 18(10):1464-1473 (2015). DOI: 10.1038/nn.4095. PubMed: 26301327.
Ramachandra, C et al. "ErbB4 Activated p38γ MAPK Isoform Mediates Early Cardiogenesis Through NKx2.5 in Human Pluripotent Stem Cells" Stem Cells 34.2:288 (2016). DOI: 10.1002/stem.2223. Application: RNA Interference.
Shimizu, T et al. "Microglia-Induced Activation of Noncanonical Wnt Signaling Aggravates Neurodegeneration in Demyelinating Disorders" Mol. Cell. Biol. 36.21:2728-2741 (2016). DOI: 10.1128/MCB.00139-16. Application: Research.
Wang, et al. "Interleukin 6 induces expression of NADPH oxidase 2 in human aortic endothelial cells via long noncoding RNA MALAT1" Die Pharmazie - An International Journal of Pharmaceutical Sciences 71.10:592 (2016). DOI: 10.1691/ph.2016.6598. Application: Transduce HAOECs.
Wei, X., Li, X., Yan, W., Zhang, X., Sun, Y., & Zhang, F. "SKP2 promotes hepatocellular carcinoma progression through nuclear AMPK-SKP2-CARM1 signaling transcriptionally regulating nutrient-deprived autophagy induction" Cellular Physiology and Biochemistry 47(6):2484-2497 (2018).
Yu, S., Ren, H., Li, Y., Liang, X., Ning, Q., Chen, X., ... & Hu, T. "HOXA4-Dependent Transcriptional Activation of AXL Promotes Cisplatin-Resistance in Lung Adenocarcinoma Cells" Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Anti-Cancer Agents) 18(14):2062-2067 (2018).
Kuo, F. et al. (2022). HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution. Cell Reports, 40(4), 111136. https://doi.org/10.1016/j.celrep.2022.111136
Bayati, A., Ayoubi, R., Aguila, A., Zorca, C. E., Han, C., Banks, E., Nguyen-Renou, E., Luo, W., Shlaifer, I., Del Cid-Pellitero, E., Yaqubi, M., Fon, E. A., Stratton, J. A., Durcan, T. M., Nahirney, P. C., & McPherson, P. S. (2023). A dual hit of α-synuclein internalization and immune challenge leads to the formation and maintenance of Lewy body-like inclusions in human dopaminergic neurons. https://doi.org/10.1101/2023.05.29.542776
Joladarashi, D., Thej, C., Mallaredy, V., Magadum, A., Cimini, M., Gonzalez, C., Truongcao, M., Nigro, J. T., Sethi, M. K., Gibb, A. A., Benedict, C., Koch, W. J., & Kishore, R. (2024). GPC3-mediated metabolic rewiring of diabetic mesenchymal stromal cells enhances their cardioprotective functions via PKM2 activation. IScience, 27(10), 111021. https://doi.org/10.1016/j.isci.2024.111021
Liang, H., Wang, B., Wang, J., Ma, B., & Zhang, W. (2022). Pyolysin of Trueperella pyogenes induces pyroptosis and IL-1β release in murine macrophages through the potassium/NLRP3/caspase-1/gasdermin D pathway. Frontiers in Immunology, 13, 832458. https://pubmed.ncbi.nlm.nih.gov/35371034/
Kuo, F.-C., Neville, M. J., Sabaratnam, R., Wesolowska-Andersen, A., Phillips, D., Wittemans, L. B. L., van Dam, A. D., Loh, N. Y., Todorčević, M., Denton, N., Kentistou, K. A., Joshi, P. K., Christodoulides, C., Langenberg, C., Collas, P., Karpe, F., & Pinnick, K. E. (2022). HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution. Cell Reports, 40(4), 111136. https://doi.org/10.1016/j.celrep.2022.111136
Yu, Y., Chen, E., Weiss, R. M., Felder, R. B., & Wei, S.-G. (2022). Transforming Growth Factor-α Acts in Hypothalamic Paraventricular Nucleus to Upregulate ERK1/2 Signaling and Expression of Sympathoexcitatory Mediators in Heart Failure Rats. Neuroscience, 483, 13–23. https://doi.org/10.1016/j.neuroscience.2021.12.030
Wang, Z., Zhou, N., Wang, W., Yu, Y., Xia, L., & Li, N. (2021). HDAC2 interacts with microRNA-503-5p to regulate SGK1 in osteoarthritis. Arthritis Research & Therapy, 23(1). https://doi.org/10.1186/s13075-020-02373-y
Qian, F., Hu, Q., Tian, Y., Wu, J., Li, D., Tao, M., Qin, L., Shen, B., & Xie, Y. (2019). ING4 suppresses hepatocellular carcinoma via a NF-κB/miR-155/FOXO3a signaling axis. International Journal of Biological Sciences, 15(2), 369–385. https://doi.org/10.7150/ijbs.28422
ABM community
Verified customer
Asked on Feb 28 2025
Answer
abm lentiviral transfer vectors use the third generation lentivirus system. It is based on the inactivated HIV genome. Note that our lentivirus packaging plasmids cannot be integrated into the host and are transiently expressed.
ABM Scientific Support
Answered on Feb 28 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
We recommend using abm’s 2nd Generation Packaging System Mix (Cat. No. LV003) or 3rd Generation Packaging System Mix (Cat. No. LV053). abm’s lentiviral vectors have been tested and are compatible with Invitrogen’s packaging mix; we have not tested other suppliers and cannot guarantee compatibility.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
Higher MOI will provide more copies of the antibiotic resistance gene per cell. Cells containing multiple copies of the resistance gene can withstand higher antibiotic concentrations compared to those at lower MOIs. The concentration of antibiotic should be adjusted to a level that will cause selection for the desired population of transduced cells without going below the minimum antibiotic concentration you have established in your killing curve.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
MOI (Multiplicity of Infection) refers to the number of viral particles per cell used in the infection, e.g. an MOI of 5 indicates that there are five infectious units (IU) or transducing units (TU) for every cell. MOI is determined by calculating the numbers of viral particles added per well then divide this number by the number of cells seeded into the well. We also recommend transducing the cells with a range of MOIs as different cell types may require different MOIs for successful transduction.
MOI = Product Titer (IU/ml) x Virus Volume (ml) / Total Cell Number
MOI = Product Titer (IU/ml) x Virus Volume (ml) / Total Cell Number
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
The concentration must be optimized for each cell type. Typical selection amounts are around 0.1 - 0.5µg per ml.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
The standard RFP used in most of our vectors is mkate2. It has an excitation wavelength of 588nm and emission wavelength of 633nm.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
These are medium-high copy plasmids and should be propagated in a cloning E. coli strain such as DH5α. Typical yields from a 250ml culture is 300-500µg plasmid DNA.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
MOI stands for multiplicity of infection. Theoretically, an MOI of 1 will provide 1 virus particle for each cell on a plate, while an MOI of 10 represents ten virus particles per cell. However, several factors can influence the optimal MOI including cell line, cell type, transduction efficiency and gene of interest. We recommend first establishing an optimal MOI for each cell line. This can be done using a range of MOIs (0, 0.5, 1, 2, 5, 10, 50) to determine the MOI required to obtain optimal gene expression
ABM Scientific Support
Answered on Mar 24 2025
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Current vector selected:
Scrambled siRNA GFP Lentivirus
Cat. No.
LVP015-G
Controls and Related Products
Susfectin™ Transfection Reagent
G4000
1.0 ml
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DNAfectin™ Plus Transfection Reagent
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2nd Generation Packaging System Mix
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200µl
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3rd Generation Packaging System Mix
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200µl
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AAViralEntry™ Transduction Enhancer (100X)
G516
1.0 ml
$190.00
ViralEntry™ Transduction Enhancer (100X)
G515
1.0 ml
$190.00
Empty
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Current vector selected:
Scrambled siRNA GFP Lentivirus
Cat. No.
LVP015-G
Controls and Related Products
Susfectin™ Transfection Reagent
G4000
1.0 ml
$245.00
DNAfectin™ Plus Transfection Reagent
G2500
1.0 ml
$245.00
2nd Generation Packaging System Mix
LV003
200µl
$293.00
3rd Generation Packaging System Mix
LV053
200µl
$355.00
AAViralEntry™ Transduction Enhancer (100X)
G516
1.0 ml
$190.00
ViralEntry™ Transduction Enhancer (100X)
G515
1.0 ml
$190.00
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abm-page-category-nav-link\u0022\u003E CRISPR Controls \u003C\/a\u003E\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/div\u003E\n\u003Cdiv id=\u0022\u0022 class=\u0022abm-container-fluid\u0022\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-md-12\u0022 style=\u0022text-align: left; padding-bottom: 1em;\u0022\u003E\n\u003Cp\u003EAt \u003Cstrong\u003Eabm\u003C\/strong\u003E, we know that every experiment requires an appropriate control. 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height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/scrambled-sirna-gfp-lentivector-lv015-g.html\u0022 rel=\u0022noopener\u0022\u003EScrambled siRNA GFP Lentivector\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 9.16127%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/scrambled-sirna-gfp-lentivector-lv015-g.html\u0022 style=\u0022outline: 0px; user-select: none;\u0022 rel=\u0022noopener\u0022\u003ELV015-G\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%; height: 22px;\u0022\u003E\u003Cspan style=\u0022color: #000000;\u0022\u003EVector\u003Cbr \/\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%;\u0022\u003E\u003Cspan style=\u0022color: #000000;\u0022\u003EPuro\u003C\/span\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 7.26849%; height: 22px;\u0022 class=\u0022product-price\u0022 data-product=\u0022LV015-G\u0022\u003E90.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 22px;\u0022\u003E\n\u003Ctd style=\u0022width: 21.2345%; 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height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/scrambled-sirna-gfp-lentivector-with-neomycin-lv015-n.html\u0022 rel=\u0022noopener\u0022\u003EScrambled siRNA GFP Lentivector with Neomycin\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 9.16127%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/scrambled-sirna-gfp-lentivector-with-neomycin-lv015-n.html\u0022 style=\u0022outline: 0px; user-select: none;\u0022 rel=\u0022noopener\u0022\u003ELV015-N\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%; height: 22px;\u0022\u003E\u003Cspan style=\u0022color: #000000;\u0022\u003EVector\u003Cbr \/\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%;\u0022\u003E\u003Cspan style=\u0022color: #000000;\u0022\u003ENeo\u003C\/span\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 7.26849%; height: 22px;\u0022 class=\u0022product-price\u0022 data-product=\u0022LV015-N\u0022\u003E90.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 22px;\u0022\u003E\n\u003Ctd style=\u0022width: 21.2345%; 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height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/scrambled-aav-sirna-control-vector-iaav01500amp.html\u0022 rel=\u0022noopener\u0022\u003EScrambled AAV siRNA Control Vector\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 9.16127%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/scrambled-aav-sirna-control-vector-iaav01500amp.html\u0022 style=\u0022outline: 0px; user-select: none;\u0022 rel=\u0022noopener\u0022\u003EiAAV01500\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%; height: 22px;\u0022\u003E\u003Cspan style=\u0022color: #000000;\u0022\u003EVector\u003Cbr \/\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%;\u0022\u003E\u003Cspan style=\u0022color: #000000;\u0022\u003EGFP-2A-Puro\u003C\/span\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 7.26849%; height: 22px;\u0022 class=\u0022product-price\u0022 data-product=\u0022iAAV01500\u0022\u003E140.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 22px;\u0022\u003E\n\u003Ctd style=\u0022width: 21.2345%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/search?query=Scrambled+AAV+siRNA+Control+Virus+\u0022 rel=\u0022noopener\u0022\u003EScrambled AAV siRNA Control Virus\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 9.16127%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/search?query=Scrambled+AAV+siRNA+Control+Virus+\u0022 style=\u0022outline: 0px; user-select: none;\u0022 rel=\u0022noopener\u0022\u003EBy Serotype\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%; height: 22px;\u0022\u003E\u003Cspan style=\u0022color: #000000;\u0022\u003EVirus\u003Cbr \/\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%;\u0022\u003E\u003Cspan style=\u0022color: #000000;\u0022\u003EGFP-2A-Puro\u003Cbr \/\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 7.26849%; height: 22px;\u0022\u003E$255.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022background-color: #f8f9fa;\u0022 align=\u0022left;\u0022 id=\u0022table-product-mini-category-LentivirusControls\u0022\u003E\n\u003Ctd style=\u0022text-align: left; width: 46.6159%; height: 22px;\u0022 colspan=\u00225\u0022\u003E\u003Cspan style=\u0022color: #00000;\u0022\u003E\u003Cstrong\u003ELentivirus Controls\u003C\/strong\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 22px;\u0022\u003E\n\u003Ctd style=\u0022width: 21.2345%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/plenti-iii-blank-vector-lv587.html\u0022 rel=\u0022noopener\u0022\u003ELenti-CMV-Blank-SV40-Puro Control Vector\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 9.16127%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 style=\u0022outline: 0px; user-select: none;\u0022 href=\u0022\/plenti-iii-blank-vector-lv587.html\u0022 rel=\u0022noopener\u0022\u003ELV587\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%; height: 22px;\u0022\u003EVector\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%;\u0022\u003EPuro\u003C\/td\u003E\n\u003Ctd style=\u0022width: 7.26849%; 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height: 44px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/lenti-cmv-gfp-2a-puro-blank-lentivirus-lvp690.html\u0022 style=\u0022outline: 0px; user-select: none;\u0022 rel=\u0022noopener\u0022\u003ELVP690\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%; height: 44px;\u0022\u003EVirus\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%;\u0022\u003EPuro\u003C\/td\u003E\n\u003Ctd style=\u0022width: 7.26849%; height: 44px;\u0022 class=\u0022product-price\u0022 data-product=\u0022LVP690\u0022\u003E205.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 22px;\u0022\u003E\n\u003Ctd style=\u0022width: 21.2345%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/plenti-ef1a-blank-vector-lv588.html\u0022 rel=\u0022noopener\u0022\u003ELenti-EF1a-Blank-SV40-Puro Control Vector\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 9.16127%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/plenti-ef1a-blank-vector-lv588.html\u0022 style=\u0022outline: 0px; 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It contains a GFP reporter and a puromycin selection marker for easy identification of transduced cells. Order online from abm and get a high-quality product with a guaranteed titer of 107 IU\/mL. The price is only $195.00 for 4 x 500 \u03bcl.","created_at":"2023-03-27 03:31:29","updated_at":"2023-12-06 18:47:03"},"gene":null}