Anti-SV40T Antibody

Cat. No.
G202
Unit
100 μg
Price
$297.00
Cat. No. G202
Name Anti-SV40T Antibody
Unit 100 μg
Clone Monoclonal
Category Monoclonal Antibodies
Application WB
Raised In Mouse
Clonality Monoclonal
Reactivity Other
Description

The Anti-SV40T Antibody is a high-affinity mouse monoclonal antibody for the specific detection of SV40 large T antigen (SV40T) in mammalian cells. Recognizing both native and denatured SV40T protein, this antibody is ideal for confirming transgene expression following SV40-mediated cell immortalization and for characterizing established SV40T-expressing cell lines. The antibody specifically recognizes SV40 large T antigen without cross-reacting with SV40 small t antigen or related polyomavirus large T antigens, ensuring confident detection of SV40T-expressing cells.

Applications:

  • Confirm SV40 large T antigen expression following cell immortalization
  • Verify successful SV40T gene delivery and stable expression
  • Quality control of newly established immortalized cell lines
  • Western blot and immunodetection of SV40T protein
Immunogen SV40 transformed mouse line B4; C-terminal
Isotype IgG2a1
Concentration 1 µg/µl
Storage Buffer PBS, pH 7.4 with 0.02% sodium azide.
Storage Condition

This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one year from date of receipt.

Formulation Liquid
Specificity The antibody reacts with native and denatured large T antigen. It does not react with small T antigen of SV40 or large T antigen of BKV and human papovavirus.
Purity Affinity purified
Guarantee

abm guarantees that all our Anti-SV40 Tag Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G202
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  • Wang, ZY et al. "Development of a cytokine-producing immortalized murine Kupffer cell line" Cytokine 2:165-172 (2014). PubMed: 25138015.
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