Anti-GAPDH Loading Control Monoclonal Antibody
Cat. No.
|
G041-1mg
Print
|
Name
|
Anti-GAPDH Loading Control Monoclonal Antibody
|
Unit
|
1.0 mg
|
Clone
|
123
|
Category
|
Loading Control Antibodies
|
Application
|
WB,IF
|
Application Info
|
test
|
Raised In
|
Mouse
|
Clonality
|
Monoclonal
|
Reactivity
|
Other
|
Description
|
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
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Isotype
|
IgG
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Concentration
|
100ug/100ul
|
Recommended Dilutions
|
It is very useful as a loading control for quantitative western blotting. The antibody recognizes a 37kDa band in a Western Blot analysis.
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Storage Buffer
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PBS, pH 7.4 with 0.05% sodium azide.
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Storage Condition
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This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
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Formulation
|
Liquid
|
Caution
|
For Research Use Only
|
Guarantee
|
abm guarantees that all our Anti-GAPDH Loading Control Monoclonal Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
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Material Citation
|
If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G041-1mg
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