Lenti-HPV E6/E7 Virus, High Titer
| Cat. No. | LV667 |
| Name | Lenti-HPV E6/E7 Virus, High Titer |
| Unit | 2 x 100 µl |
| Unpacking and Storage Instructions |
Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles. |
| Description |
High Titer (109 IU/ml) Recombinant Lentivirus expressing the HPV-16 E6/E7 gene. |
| Application |
Cell immortalization of keratinocytes. |
| Storage Condition | Dry Ice |
| Shipping Conditions | Dry Ice |
| Expression System Type | Lentivirus |
| Caution |
This product is distributed for laboratory research only. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. LV667 |
Print/Download Datasheet
| What is the general procedure for immortalizing cells? | |
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See our Cell Immortalization Handbook for more details.
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| Can you recommend a specific reagent and protocol to immortalize my cell line? | |
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Successful cell immortalization is unpredictable and must be determined experimentally, as results vary with species, tissue origin, growth characteristics and whether or not specific cell functions need to be preserved.
Our in-house experiments using the SV40T and hTERT lentiviruses have achieved the highest immortalization success rates in a variety of different cell types. |
| Do immortalized cells retain all characteristics of the original primary cell line? | |
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Not always. Some phenotypic or genetic changes may occur during immortalization. We recommend using our hTERT immortalization reagents as this method preserves phenotype and karyotype as well as minimizes genomic instability.
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| Do I need a control when performing cell immortalization experiments? | |
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Yes. Always include control cells that are not transduced in order to compare growth rates and detect senescence bypass.
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| How do I confirm successful cell immortalization? | |
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Assess long-term growth beyond normal passage limits and verify expression of the immortalizing genes via PCR, qPCR or Western blot.
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| Where can I learn more about cell immortalization? | |
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Check out our Learning Resources and Youtube video for more information.
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| How do the E6/E7 genes immortalize cells? | |
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The oncogenes E6 and E7 from HPV work by targeting the cell's tumor suppressor pathways (p53 and Rb) effectively over riding senescence and apoptosis checkpoints.
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| Will this virus immortalize my primary cells? | |
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This is highly dependent on the immortalization reagent and cell type. Check out our Cell Immortalization Reagents Compatibility Chart for more information.
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- Balducci, L., Blasi, A., Saldarelli, M., Soleti, A., Pessina, A., Bonomi, A., Coccè, V., Dossena, M., Tosetti, V., Ceserani, V., Navone, S., Falchetti, M., Parati, E., & Alessandri, G. (2014). Immortalization of human adipose-derived stromal cells: production of cell lines with high growth rate, mesenchymal marker expression and capability to secrete high levels of angiogenic factors. Stem Cell Research & Therapy, 5(3), 63. https://doi.org/10.1186/scrt452
- Coleman, S., Choi, K. Y., Root, M., & McGregor, A. (2016). A Homolog Pentameric Complex Dictates Viral Epithelial Tropism, Pathogenicity and Congenital Infection Rate in Guinea Pig Cytomegalovirus. PLOS Pathogens, 12(7), e1005755. https://doi.org/10.1371/journal.ppat.1005755
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