Lenti-hTERT Virus

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Immortalizing your cell line will eliminate the frequent need to re-establish fresh cultures from specimens. Immortalized cell lines are capable of extended proliferation and should possess similar or identical genotype and phenotype to the parental tissue. \u003Cbr \/\u003E\u003Cbr \/\u003E\u003Cstrong\u003EImmortalization Strategies for Mammalian Cells\u003C\/strong\u003E\u003Cbr \/\u003ESeveral methods exist for immortalizing mammalian cells in culture. With years of experience, \u003Cstrong\u003Eabm\u003C\/strong\u003E has developed the most comprehensive cell immortalization products comprising of ready-to-use retroviral, lentiviral and adenoviral vectors.\u003Cbr \/\u003E\u003Cbr \/\u003E\u003C\/p\u003E\n\u003Cdiv\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table2\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth\u003EGene Overexpressed\u003C\/th\u003E\n\u003Cth\u003EViruses Offered\u003C\/th\u003E\n\u003Cth\u003EApplication\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EhTERT\u003C\/td\u003E\n\u003Ctd\u003EAdenovirus,\u003Cbr \/\u003ELentivirus,\u003Cbr \/\u003ERetrovirus\u003C\/td\u003E\n\u003Ctd\u003EReliable and commonly used.\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003ESV40 T Antigen\u003C\/td\u003E\n\u003Ctd\u003EAdenovirus,\u003Cbr \/\u003ELentivirus,\u003Cbr \/\u003ERetrovirus\u003C\/td\u003E\n\u003Ctd\u003EReliable and commonly used. Often used to immortalize epithelial cells.\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EEBV genes\u003C\/td\u003E\n\u003Ctd\u003EEpstein-Barr Virus\u003C\/td\u003E\n\u003Ctd\u003EImmortalize B lymphocytes, and sometimes T lymphocytes.\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EHPV E6-E7\u003C\/td\u003E\n\u003Ctd\u003ELentivirus\u003C\/td\u003E\n\u003Ctd\u003EImmortalize keratinocytes.\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EHOXB8, HOXA9, HOXA10\u003C\/td\u003E\n\u003Ctd\u003ELentivirus\u003C\/td\u003E\n\u003Ctd\u003EImmortalize hematopoietic cells, including macrophages, hematopoetic progenitor cells, and myeloid progenitor cells.\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EAdenoviral E1A-E1B\u003C\/td\u003E\n\u003Ctd\u003ELentivirus\u003C\/td\u003E\n\u003Ctd\u003EImmortalize a wide range of primary rodent cells, including amniotic fluid cells, primary epithelial cells, fibroblasts, and primary kidney cells.\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003Ep53 siRNA, Rb siRNA\u003C\/td\u003E\n\u003Ctd\u003ELentivirus\u003C\/td\u003E\n\u003Ctd\u003ECo-express with hTERT for better immortalization of epithelial cells.\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EBmi1\u003C\/td\u003E\n\u003Ctd\u003ELentivirus\u003C\/td\u003E\n\u003Ctd\u003EImmortalize epithelial cells.\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003ECDK4\u003C\/td\u003E\n\u003Ctd\u003ELentivirus\u003C\/td\u003E\n\u003Ctd\u003ECo-express with hTERT for a more authentic cell model.\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EMyc T58A (c-Myc), Ras V12 (H-Ras)\u003C\/td\u003E\n\u003Ctd\u003ELentivirus\u003C\/td\u003E\n\u003Ctd\u003EImmortalizes a wide variety of cells, including neural stem cells, primary kidney cells, erythroblasts, and a variety of epithelial cells (primary prostate, mammary, airway, etc.).\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-md-4 col-sm-4 col-lg-4 col-xs-12\u0022\u003E\n\u003Cdiv class=\u0022mini-box2\u0022\u003E\u003Cspan class=\u0022bluetext\u0022\u003E\u003Cb\u003EPopular Products\u003C\/b\u003E\u003C\/span\u003E\n\u003Cul class=\u0022grey-list grey-link\u0022\u003E\n\u003Cli\u003E\u003Ca class=\u0022abm-category-list-cname\u0022 href=\u0022\/viralplus-transduction-enhancer-g698.html\u0022\u003EViralPlus Transduction Enhancer\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Ca class=\u0022abm-category-list-cname\u0022 href=\u0022\/gfp-control-lentivirus-neo-lv006.html\u0022\u003EGFP Control Lentivirus (Neo)\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Ca class=\u0022abm-category-list-cname\u0022 href=\u0022\/pricoat-flasks.html#product-list\u0022\u003EPriCoat Flasks\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Ca class=\u0022abm-category-list-cname\u0022 href=\u0022\/lenti-htert-no-selection-marker-virus.html\u0022\u003E\u003Cspan\u003ELenti-hTERT (No Selection Marker) virus\u003C\/span\u003E\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Ca class=\u0022abm-category-list-cname\u0022 href=\u0022\/Immortalized-Cell-Lines.html\u0022\u003EImmortalized Cells\u003C\/a\u003E\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003Cspan class=\u0022bluetext\u0022\u003E\u003Cb\u003EAdditional Resources on Cell Culture Methods\u003C\/b\u003E\u003C\/span\u003E\n\u003Cul class=\u0022grey-list grey-link\u0022\u003E\n\u003Cli\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/cell-culture-introduction\u0022 rel=\u0022noopener\u0022\u003ECell Culture - An Introduction\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/growth-factors-cytokines-introduction\u0022 rel=\u0022noopener\u0022\u003EGrowth Factors and Cytokines - An Introduction\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/lentivirus-system-introduction\u0022 rel=\u0022noopener\u0022\u003EThe Lentivirus - An Introduction\u003C\/a\u003E\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv id=\u0022abm-category-section4\u0022 class=\u0022abm-container-fluid\u0022\u003E\n\u003Ch3\u003ETools \u0026amp; Resources\u003C\/h3\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022module abm-category-module\u0022 style=\u0022margin-top: 3em;\u0022\u003E\n\u003Cul class=\u0022htmlcontent-home\u0022\u003E\n\u003Cli class=\u0022col-sm-4 col-md-4\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv class=\u0022abm-category-workflow\u0022\u003E\u003Ca href=\u0022\/assets\/images\/category\/cell_biology\/Cell_Immortalization_Handbook_V7.pdf\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/category\/cell_immort_kits\/CRISPR-handbook.png\u0022 alt=\u0022Cell Immortalization Handbook\u0022 \/\u003E\u003C\/a\u003E \u003Cbr \/\u003E\n\u003Cdiv class=\u0022abm-sub-category-image-title\u0022\u003E\u003Cstrong\u003ECell Immortalization Handbook\u003C\/strong\u003E \u003Cbr \/\u003E\u003Cspan\u003EThis Handbook is designed to give an overview of Cell Immortalization, Including methods, protocols, and reagents.\u003C\/span\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003Cli class=\u0022col-sm-4 col-md-4\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv class=\u0022abm-category-workflow\u0022\u003E\u003Ca\u003E\u003Cimg src=\u0022\/assets\/images\/category\/cell_immort_kits\/Cell-Immortalization-Workflow-2png\u0022 alt=\u0022Cell Immortalization Workflow \u0022 \/\u003E\u003C\/a\u003E\u003Cbr \/\u003E\n\u003Cdiv class=\u0022abm-sub-category-image-title\u0022\u003E\u003Cstrong\u003ECell Immortalization Workflow \u003C\/strong\u003E \u003Cbr \/\u003E\u003Cspan\u003EOverview of \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s Immortalization service workflow. 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