pShuttle-HA
| Cat. No. | A009 |
| Name | pShuttle-HA |
| Unit | 5.0 µg |
| Description |
The Adeno-2™ Adenovirus Expression System provides reagents for the efficient construction of recombinant adenoviral vectors with your expression cassette being HA or His tagged. This facilitates detection of your transgene in target cells. The system also provides either pShuttle-His and pShuttle-HA vectors with multiple cloning sites for convenient subcloning. |
| Vector | pShuttle-HA |
| Note |
NOT FOR RESALE without prior written consent of ABM. This product is distributed for laboratory research only. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. A009 |
Print/Download Datasheet
| Can this kit purify all adenoviruses into the titer level at least 10^11 pfu/mL? | |
|
Yes, if the insert gene in adenovirus is not toxic to cells. there are no difference in performance as regards to different kit and the difference is in the different amount of elution (final amount). However, high titer can be further concentrated with our buffer exchange kit.
|
| Would you have the titer data regarding adenoviruses that are lac g or cre recombinase gene-injected? | |
|
We have in house experience with these two genes and they are not toxic, so higher titer adenovirus can be prepared.
|
| I have a question regarding preparation adenovirus stock for in vivo use. Adenoviral storage buffer in Viral stock buffer exchange kit (Cat.No.G130) is composed of 2.5% glycerol etc. which make it hard to inject in vivo in general. Accoriding to FAQs, 10mM Tris(pH8.0)plus 4% sucrose seems to be better formulation, but how can I remove 2.5% glyserol etc. from adenoviral storage buffer? How can buffer be exchanged from adenoviral storage buffer(Cat.No.G130) to 10mM Tris, 4% sucrose? | |
|
In that case, you will have to go through the buffer exchange kit again using the sucrose buffer. A 3x20ml exchange will do the job.
|
| What are viral particle (VP), plaque formation unit (PFU), and infectious unit (IFU)? | |
|
Viral particles (VPs) represent the total number of viral particles (infectious and infection-deficient combined). Due to variations in virus preparations, the ratio of infectious /non-infectious varies significantly and therefore, VP does not reflect the concentration of virus in a preparation. PFU (plaque forming unit) represents the number of infectious or live viruses. It reflects the concentration of infectious viruses in a preparation. IFU (infectious unit) is biologically equivalent to PFU. For most virus preps, the VP/PFU ratio is 20:1 to 50:1.
|
| How are virus titers determined? | |
|
There are 3 commonly used protocols for determining adenovirus titer: (1) OD260 Assay, (2) Plaque Formation Assay, and (3) End-point Dilution Assay.
|
| How does TCID50 work? | |
|
Please see the link before for the assay. TCID50 is the same as end dilution assay.
http://www.abmgood.com/TechSupport/adeno-vec.php
If the customer provide us the virus, we can provide its titer with the Cat#C008 service.
|
| Is this adenoviral gene transfer technique available for in vivo use? Is it OK to inject via tail vein? | |
|
Yes, but higher titers up 1x10e12 pfu/ml have to be used. We do offer custom adenovirus amplification and purification.
|
| I'm wanting to overexpress my interest protein in mouse liver. Does this adenovirus transfer the gene specifically to the liver? | |
|
Over 95% of adenoviral vectors will end up in liver if you inject in the blood stream.
|
| I'd like to perform a buffer exchange, but your Buffer Exchange column Cat# G130 seems to be discontinued. Can you offer me any recommendations for doing this? | |
|
Unfortunately yes, our Buffer Exchange column G130 has now been discontinued due to a lack of supply for the filter component. In order to run your own buffer exchange you will require: 50K molecular weight cut off low protein binding membrane column (ensuring it will hold up to 15 ml) with a suggested buffer recipe as follows: 1 X PBS buffer, pH 7.2
|
- Siu, CW et al. "Modeling of lamin A/C mutation premature cardiac aging using patient-specific induced pluripotent stem cells" Aging (Albany NY) 4(11):803-822 (2012).
- Mcallister, J. M., Strauss, J. F., & Christensen, N. D. "U" S. Patent Application No. 16/201 360: (2019).
- Qimuge, N., He, Z., Qin, J., Sun, Y., Wang, X., Yu, T., … Pang, W. "Overexpression of DNMT3A promotes proliferation and inhibits differentiation of porcine intramuscular preadipocytes by methylating p21 and PPARg promoters" Gene 696:54–62 (2019). DOI: 10.1016/j.gene.2019.02.029.
This product has no review yet.
Controls and Related Product:
{"id":438,"cat_no":"A009","sku_in_m2":"","old_cat_no":"A009","name":"pShuttle-HA","category_id":76,"category_name":"Cloning Vectors \u0026 Packaging Mixes","frontend_category_id":75,"frontend_category_ids":"86,57,72,75","gene_id":0,"gene_name":"","gene_full_name":"","alias":"","unit_quantity":"5.0 \u0026micro;g","accession_number":"","species_id":0,"species":"","raised_in_id":0,"raised_in":"","cell_type_id":0,"cell_type":"","tissue_id":0,"tissue":"","price":"375.0000","price_before_discount":"0.0000","keywords":"adeno vectors and cloning kits","invisible_search_keywords":"","filter_group_id":34,"filter_group_name":"Molecular Biology","filter_id":66,"filter_name":"Cloning Vectors \u0026 Packaging Mixes","search_filter_ids":"55,57","search_filter_keys":"55-57","temp_field":null,"customization":0,"filter_table_id":17187083,"filter_table_name":"","related_group_id":16,"url":"","operator":"","points_rule":6,"status":1,"need_remove":0,"document_sort":"","price_old":"350.0000","price2024":"365.0000","created_at":null,"updated_at":"2025-01-06 08:31:49","Confirmed":"Tony Confirmed","geo_price_before_discount":"0.00","geo_titer7_price_before_discount":"149.00","cate_url":"Cloning-Vectors-Packaging-Mixes.html","frontendBreadcrumbs":[{"id":57,"pid":86,"name":"Genetic Materials","page_id":8,"page_type":"App\\Models\\Category","href":null,"sort":57,"show_in_nav":1,"show_in_homepage":1,"has_children":1,"status":1,"related_table":null,"created_at":null,"updated_at":"2025-01-20 08:46:24","url_key":"genetic-materials.html","url":"genetic-materials.html"},{"id":72,"pid":57,"name":"Expression Systems","page_id":192,"page_type":"App\\Models\\Category","href":null,"sort":72,"show_in_nav":1,"show_in_homepage":1,"has_children":1,"status":1,"related_table":null,"created_at":null,"updated_at":null,"url_key":"expression-systems.html","url":"expression-systems.html"},{"id":75,"pid":72,"name":"Adenoviral Vectors","page_id":211,"page_type":"App\\Models\\Cms","href":null,"sort":75,"show_in_nav":1,"show_in_homepage":1,"has_children":0,"status":1,"related_table":null,"created_at":null,"updated_at":null,"url_key":"recombinant-adenoviral-vectors","url":"recombinant-adenoviral-vectors"}],"price_flag":0,"builderURL":"","builderViewerURL":"\/vds\/viewer\/cat\/142?embed=1","mapURL":"\/vds\/map\/cat\/142","viewerURL":"\/vds\/viewer\/cat\/142","builderType":"","productType":"App\\Models\\CatalogBaseMolecular","geo_price":"375.00","geo_price_symbol":"$375.00","category":{"id":76,"categories":"Cloning Vectors \u0026 Packaging Mixes","pid":27,"url_key":"Cloning-Vectors-Packaging-Mixes.html","css":null,"wid":0,"breadcrumbs":"[{\u0022url\u0022:\u0022https:\/www.abmgood.com\/\u0022,\u0022title\u0022:\u0022Home\u0022},{\u0022url\u0022:\u0022https:\/www.abmgood.com\/general-materials.html\u0022,\u0022title\u0022:\u0022General Materials \u0026amp; Others\u0022},{\u0022url\u0022:\u0022https:\/\/www.abmgood.com\/Cloning-Vectors-Packaging-Mixes.html\u0022,\u0022title\u0022:\u0022Cloning Vectors \u0026amp; Packaging Mixes\u0022}]","breadcrumbsIdJson":"\u003Cscript type=\u0022application\/ld+json\u0022\u003E{\u0022@context\u0022:\u0022https:\/\/schema.org\u0022,\u0022@type\u0022:\u0022BreadcrumbList\u0022,\u0022itemListElement\u0022:[{\u0022@type\u0022:\u0022ListItem\u0022,\u0022position\u0022:1,\u0022name\u0022:\u0022Home\u0022,\u0022item\u0022:\u0022https:\/www.abmgood.com\/\u0022},{\u0022@type\u0022:\u0022ListItem\u0022,\u0022position\u0022:2,\u0022name\u0022:\u0022General Materials \u0026amp; Others\u0022,\u0022item\u0022:\u0022https:\/www.abmgood.com\/general-materials.html\u0022},{\u0022@type\u0022:\u0022ListItem\u0022,\u0022position\u0022:3,\u0022name\u0022:\u0022Cloning Vectors \u0026amp; Packaging Mixes\u0022}]}\u003C\/script\u003E","breadcrumbsHtml":"\u003Cul class=\u0022abm-breadcrumb\u0022\u003E\u003Cli class=\u0022breadcrumb-item\u0022\u003E\u003Ca href=\u0022https:\/www.abmgood.com\/\u0022\u003EHome\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022breadcrumb-item\u0022\u003E\u003Ca href=\u0022https:\/www.abmgood.com\/general-materials.html\u0022\u003EGeneral Materials \u0026amp; Others\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022active breadcrumb-item\u0022\u003ECloning Vectors \u0026amp; Packaging Mixes\u003C\/li\u003E\u003C\/ul\u003E","description":"\u003Cul class=\u0022abm-breadcrumb\u0022\u003E\n\u003Cli class=\u0022abm-text-gray\u0022\u003E\u003Ca style=\u0022font-weight: bold; text-decoration: none; color: #808083;\u0022 href=\u0022\/\u0022\u003EHome\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Ca style=\u0022font-weight: bold; text-decoration: none; color: black;\u0022 href=\u0022\/general-materials.html\u0022\u003EGeneral Materials \u0026amp; Others\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003ECloning Vectors \u0026amp; Packaging Mixes\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003Ch1 id=\u0022abm-category-home-title\u0022\u003ECloning Vectors \u0026amp; Packaging Mixes\u003C\/h1\u003E\n\u003Cdiv id=\u0022abm-category-section1\u0022 class=\u0022abm-container-fluid\u0022\u003E\u003C!--\u003Ch3\u003EOverview\u003C\/h3\u003E--\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-md-8\u0022 style=\u0022text-align: left; padding-bottom: 3em;\u0022\u003E\u003Cb\u003Eabm\u003C\/b\u003E is a world leader in recombinant viral technology, providing a comprehensive collection of lentiviral, adenoviral and retroviral vectors, in addition to viral packaging mixes and purification kits. We offer a variety of viral cloning vectors containing multiple cloning site for your specific insert. These vectors also come with various different promoters, reporters and tags for all your experimental needs.\u0026nbsp;\u003Cbr \/\u003E\u003Cbr \/\u003ECloning not your expertise? We\u0027ve got you covered with our \u003Cspan style=\u0022color: #ef6331;\u0022\u003E\u003Ca href=\u0022\/Custom-Cloning.html\u0022 style=\u0022color: #ef6331;\u0022\u003ECustom Cloning Service\u003C\/a\u003E\u003C\/span\u003E where we can clone your desired vector construct for you.\u0026nbsp;\u003C\/div\u003E\n\u003Cdiv class=\u0022col-md-4 col-sm-4 col-lg-4 col-xs-12\u0022 style=\u0022margin-top: 0;\u0022\u003E\n\u003Cdiv class=\u0022mini-box2\u0022\u003E\u003Cspan class=\u0022bluetext\u0022\u003E\u003Cb\u003EPopular Products\u003C\/b\u003E\u003C\/span\u003E\n\u003Cul class=\u0022grey-list grey-link\u0022\u003E\n\u003Cli\u003E\u003Ca href=\u0022\/2nd-generation-packaging-system-mix-lv003.html\u0022\u003E2nd Generation Lentivirus Packaging Mix\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Ca href=\u0022\/3rd-generation-packaging-system-mix-lv053.html\u0022\u003E3rd Generation Lentivirus Packaging Mix\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Ca href=\u0022\/speedy-lentivirus-purification-lv999.html\u0022\u003ESpeedy Lentivirus Purification\u003C\/a\u003E\u0026nbsp;\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv id=\u0022abm-category-section4\u0022 class=\u0022abm-container-fluid\u0022\u003E\n\u003Ch3\u003EProducts\u003C\/h3\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022module abm-category-module col-md-12\u0022\u003E\n\u003Cul class=\u0022htmlcontent-home\u0022\u003E\n\u003Cli class=\u0022col-sm-4 col-md-4\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv\u003E\u003Ca href=\u0022\/search?query=lentiviral+vectors+and+cloning+kits\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/category\/cloning_vectors_packaging_mixes\/Lentiviral-vectors-\u0026amp;-cloning-kits.png\u0022 alt=\u0022Lentiviral-vectors-\u0026amp;-cloning-kits\u0022 width=\u0022100%\u0022 \/\u003E \u003Cbr \/\u003E\u003C\/a\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003Cli class=\u0022col-sm-4 col-md-4\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv\u003E\u003Ca href=\u0022\/search?query=adeno+vectors+and+cloning+kits\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/category\/cloning_vectors_packaging_mixes\/adenovectors-\u0026amp;-cloning-kits.png\u0022 alt=\u0022adenovectors-\u0026amp;-cloning-kits\u0022 width=\u0022100%\u0022 \/\u003E \u003Cbr \/\u003E\u003C\/a\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003Cli class=\u0022col-sm-4 col-md-4\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv\u003E\u003Ca href=\u0022\/search?query=retroviral+vectors+and+cloning+kits\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/category\/cloning_vectors_packaging_mixes\/retroviral-vectors-\u0026amp;-cloning-kits.png\u0022 alt=\u0022retroviral-vectors-\u0026amp;-cloning-kits\u0022 width=\u0022100%\u0022 \/\u003E \u003Cbr \/\u003E\u003C\/a\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003Cli class=\u0022col-sm-4 col-md-4\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv\u003E\u003Ca href=\u0022\/search?query=lentivirus+packaging+mixes\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/category\/cloning_vectors_packaging_mixes\/lentivirus-packaging-mixes.png\u0022 alt=\u0022lentivirus-packaging-mixes\u0022 width=\u0022100%\u0022 \/\u003E \u003Cbr \/\u003E\u003C\/a\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003Cli class=\u0022col-sm-4 col-md-4\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv\u003E\u003Ca href=\u0022\/search?query=aav+packaging+mixes\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/category\/cloning_vectors_packaging_mixes\/aav-packaging-mixes-\u0026amp;-serotypes.png\u0022 alt=\u0022aav-packaging-mixes-\u0026amp;-serotypes\u0022 width=\u0022100%\u0022 \/\u003E \u003Cbr \/\u003E\u003C\/a\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003Cli class=\u0022col-sm-4 col-md-4\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv\u003E\u003Ca href=\u0022\/search?query=virus+purification+kits\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/category\/cloning_vectors_packaging_mixes\/virus-purification-kits.png\u0022 alt=\u0022virus-purification-kits\u0022 width=\u0022100%\u0022 \/\u003E \u003Cbr \/\u003E\u003C\/a\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022module abm-category-module col-md-12\u0022\u003E\n\u003Cdiv class=\u0022col-sm-4 col-md-4\u0022\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-4 col-md-4\u0022\u003E\n\u003Cp\u003E\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv id=\u0022abm-category-section3\u0022 class=\u0022abm-container-fluid\u0022\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022module abm-category-module col-md-12\u0022\u003E\n\u003Cdiv class=\u0022col-sm-4 col-md-4\u0022\u003E\n\u003Cp class=\u0022orange-suttitle\u0022\u003E\u003Cstrong\u003ELentivirus Packaging Mixes\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cul class=\u0022product-list\u0022\u003E\n\u003Cli\u003E\u003Ca href=\u0022\/2nd-generation-packaging-system-mix-lv003.html\u0022\u003ELentivirus Packaging System Mix (2nd Generation)\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Ca href=\u0022\/3rd-generation-packaging-system-mix-lv053.html\u0022\u003ELentivirus Packaging System Mix (3rd Generation)\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Ca href=\u0022\/lentivirus-kit-lv098.html\u0022\u003ELentivirus Packaging Kit\u003C\/a\u003E\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-4 col-md-4\u0022\u003E\n\u003Cp class=\u0022orange-suttitle\u0022\u003E\u003Cstrong\u003EAAV Packaging Mixes\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cul class=\u0022product-list\u0022\u003E\n\u003Cli\u003E\u003Ca href=\u0022\/aav-packaging-mix-serotype-1-aav1001.html\u0022\u003EAAV Packaging Mix (Serotype 1)\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Ca href=\u0022\/aav-packaging-mix-serotype-2-aav1002.html\u0022\u003EAAV Packaging Mix (Serotype 2)\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Ca href=\u0022\/aav-packaging-mix-serotype-3-aav1003.html\u0022\u003EAAV Packaging Mix (Serotype 3)\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Ca href=\u0022\/aav-packaging-mix-serotype-4-aav1004.html\u0022\u003EAAV Packaging Mix (Serotype 4)\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Ca href=\u0022\/aav-packaging-mix-serotype-5-aav1005.html\u0022\u003EAAV Packaging Mix (Serotype 5)\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Ca href=\u0022\/aav-packaging-mix-serotype-6-aav1006.html\u0022\u003EAAV Packaging Mix (Serotype 6)\u003C\/a\u003E\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-4 col-md-4\u0022\u003E\n\u003Cp class=\u0022orange-suttitle\u0022\u003E\u003Cstrong\u003EVirus Purification Kits\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cul class=\u0022product-list\u0022\u003E\n\u003Cli\u003E\u003Ca href=\u0022\/speedy-lentivirus-purification-lv999.html\u0022\u003ESpeedy Lentivirus Purification Kit\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Ca href=\u0022\/ultra-pure-lentivirus-purification-kit-lv998.html\u0022\u003EUltra-Pure Lentivirus Purification Kit\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Ca href=\u0022\/add-n-pure-trade-adenovirus-purification-kit-a905.html\u0022\u003EAdd-N-Pure\u003Cspan style=\u0022font-family: \u0027open sans\u0027; font-size: 14px; background-color: #ffffff;\u0022\u003E\u0026trade;\u003C\/span\u003E\u0026nbsp;Adenovirus Purification Kit\u003C\/a\u003E\u0026nbsp;\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv id=\u0022abm-category-section5\u0022 class=\u0022abm-container-fluid\u0022\u003E\n\u003Ch3\u003EResources\u003C\/h3\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022module abm-category-module col-md-12\u0022\u003E\n\u003Cul class=\u0022htmlcontent-home\u0022\u003E\n\u003Cli class=\u0022col-sm-4 col-md-4 col-xs-6\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/lentivirus-system\u0022\u003E \u003Cimg src=\u0022\/uploads\/images\/other\/learning\/lentivirus-system-learning-resources-icon.webp\u0022 alt=\u0022Viral Expression Systems Brochure\u0022 width=\u0022100%\u0022 \/\u003E \u003Cbr \/\u003E\u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EThe Lentivirus\u003C\/strong\u003E \u003Cbr \/\u003E\u003Ci\u003ELearning resources\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003Cli class=\u0022col-sm-4 col-md-4 col-xs-6\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/adeno-associated-virus-aav\u0022 rel=\u0022noopener\u0022\u003E\u003Cimg src=\u0022\/uploads\/images\/other\/learning\/adeno-associated-virus-aav-learning-resources-icon.webp\u0022 width=\u0022100%\u0022 alt=\u0022piLenti-siRNA-GFP2 vector map\u0022 \/\u003E\u003Cbr \/\u003E\u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EThe Adeno Associated Virus(AAV)\u003C\/strong\u003E \u003Cbr \/\u003E\u003Cem\u003ELearning resources\u003C\/em\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003Cli class=\u0022col-sm-4 col-md-4 col-xs-6\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/adenovirus-system\u0022 rel=\u0022noopener\u0022\u003E\u003Cimg src=\u0022\/uploads\/images\/other\/learning\/adenovirus-system-learning-resources-icon.webp\u0022 width=\u0022100%\u0022 alt=\u0022Lenti-siRNA Expression Systems\u0022 \/\u003E\u003Cbr \/\u003E\u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EThe Adenovirus\u003C\/strong\u003E \u003Cbr \/\u003E\u003Cem\u003ELearning resources\u003C\/em\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003Cli class=\u0022col-sm-4 col-md-4 col-xs-6\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv class=\u0022abm-category-workflow\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/category\/lenti_expres_syst\/Lentiviral-Expression-System-Summary.png\u0022 alt=\u0022Available Lentivirus Expression Systems\u0022 width=\u0022100%\u0022 \/\u003E \u003Cbr \/\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EAvailable Lentivirus Systems\u003C\/strong\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003Cli class=\u0022col-sm-4 col-md-4 col-xs-6\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv class=\u0022abm-category-workflow\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/category\/adenovirus_expression_system\/Adeno-Expression-System-Summary-Table.png\u0022 alt=\u0022Available Lentivirus Expression Systems\u0022 width=\u0022100%\u0022 \/\u003E \u003Cbr \/\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EAvailable Adenovirus Systems\u003C\/strong\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003Cli class=\u0022col-sm-4 col-md-4 col-xs-6\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv class=\u0022abm-category-workflow\u0022\u003E\u003Ca href=\u0022\/assets\/catalog\/category\/docs\/v\/i\/Viral-Vectors-Brochure-Single-Pages.pdf\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/category\/wysiwyg\/VES.jpg\u0022 alt=\u0022Available Lentivirus Expression Systems\u0022 width=\u0022100%\u0022 gt=\u0022\u0022 br=\u0022\u0022 \/\u003E\u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EViral Vectors Brochure\u003C\/strong\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Ch3\u003E\u003Cstrong\u003ECitations\u003C\/strong\u003E\u003C\/h3\u003E\n\u003Cdiv\u003E\n\u003Ctable\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Cspan class=\u0022citations-num\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=10.1038\/ncomms9371\u0022\u003E01\u003C\/a\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003Ctd\u003ELenzini, S. et al. (2020). \u0022Matrix mechanics and water permeation regulate extracellular vesicle transport.\u0022 Nature Nanotechnology. 15, 217-223. \u003Cspan style=\u0022color: #ef6331;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022https:\/\/www.nature.com\/articles\/s41565-020-0636-2\u0022 style=\u0022color: #ef6331;\u0022\u003Edoi: 10.1038\/s41565-020-0636-2\u003C\/a\u003E. \u003Cspan style=\u0022color: #000000;\u0022\u003EProduct: 2nd Generation Packaging System Mix (Cat.No LV003).\u003C\/span\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Cspan class=\u0022citations-num\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=10.1038\/ncomms9371\u0022\u003E02\u003C\/a\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003Ctd\u003EPapes, F. et al. (2022). \u0022Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content.\u0022 Nature Communications. 13(1).\u0026nbsp; \u003Cspan style=\u0022color: #ef6331;\u0022\u003E\u003Ca href=\u0022https:\/\/www.nature.com\/articles\/s41467-022-29942-w\u0022 style=\u0022color: #ef6331;\u0022\u003Edoi: 10.1038\/s41467-022-29942-w\u003C\/a\u003E\u003Ca href=\u0022https:\/\/www.nature.com\/articles\/s41467-022-29942-w\u0022\u003E.\u003C\/a\u003E \u003Cspan style=\u0022color: #000000;\u0022\u003EProduct: pLenti-Promoterless Vector (Cat.No LV013).\u003C\/span\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Cspan class=\u0022citations-num\u0022\u003E03\u003C\/span\u003E\u003C\/td\u003E\n\u003Ctd\u003ECho, I et al. (2022). Deterministic Single Cell Encapsulation in Asymmetric Microenvironments to Direct Cell Polarity. Advanced Science. \u003Cspan style=\u0022color: #ef6331;\u0022\u003E\u003Ca href=\u0022https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/advs.202206014\u0022 style=\u0022color: #ef6331;\u0022\u003Edoi: 10.1002\/advs.202206014.\u003C\/a\u003E\u003C\/span\u003E Product: 2nd Generation Packaging System Mix (Cat.No LV003).\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E","meta_title":"Cloning Vectors \u0026 Packaging Mixes","meta_keywords":"viral packaging mix,lentivirus packaging mix,aav packaging mix,lentivirus packaging plasmids,aav packaging plasmids,aav serotype plasmids,lentiviral cloning vectors,aav,2nd generation lentivirus packaging mix,3rd generation lentivirus packaging mix,shuttle vectors,cloning vectors,viral cloning vectors","meta_description":"abm offers a variety of cloning vectors containing multiple cloning site for your specific insert. These vectors also come with various different promoters, reporters and tags for all your experimental needs.\u00a0","deleted_at":null,"enable":"Y","parent_list":"27","table_name":null,"image":null,"independentPage":0,"top_type":1,"sort_order":114,"in_footer":1,"fid":27,"created_at":null,"updated_at":"2025-04-02 01:04:45"},"info":{"id":16433,"cat_no_base":"A009","parent_id":438,"description":"\u003Cp\u003EThe Adeno-2\u0026trade; Adenovirus Expression System provides reagents for the efficient construction of recombinant adenoviral vectors with your expression cassette being HA or His tagged. This facilitates detection of your transgene in target cells. The system also provides either pShuttle-His and pShuttle-HA vectors with multiple cloning sites for convenient subcloning.\u003C\/p\u003E","disclaimer":"\u003Ca href=\u0022\/terms#disclaimer-section\u0022\u003EClick for more info\u003C\/a\u003E","application":null,"components":null,"cas9_origin":null,"concentration":null,"enzymes_size":null,"genecraft_series":null,"guarantee":"","population":null,"qc":null,"format_general":null,"including_screening_kit":null,"expression_system_general":null,"purity":null,"image":null,"insert_size":null,"shipping_conditions":null,"source_catalog_number":null,"inactivation_protocol":null,"led_viewer_compatibility":null,"unit_definition":null,"vector":"pShuttle-HA","reaction_buffer":null,"storage_buffer":null,"caution":"Not for diagnostic use.","storage_condition":null,"product_volume":null,"reporter":null,"safeview_series":null,"source_catno":"Jennie","stain_color":null,"supplier":"ABM","internal_supplier":null,"internal_note":null,"inventory_location":"pre-made lentivirus39,J8","note":"\u003Cp\u003ENOT FOR RESALE without prior written consent of ABM. This product is distributed for laboratory research only.\u003C\/p\u003E","recommend":null,"depositor":null,"licensor_name":null,"licensor_contact_information":null,"contract_termination_date":null,"royalty_rates":null,"cas_type":null,"cas_origin":null,"cas_protein_marker":null,"source":null,"endotoxin_level":null,"additional_information":null,"titer":null,"nucleotide_format":null,"protocol_overview":null,"source_price":null,"created_at":"2022-09-09 05:48:46","updated_at":"2025-03-09 19:03:44","short_description":null,"reaction_definition":null,"specificity":null,"promotions":null},"maps":[{"id":142,"name":"pShuttle-HA","sequence":"ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgtgggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaacacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacaggaagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagtaagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttgtgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggtagcgaaagctcagatctggatctcccgatcccctatggtcgactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgcgatgtacgggccagatatacgcgttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggaaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagagctagccttaagaagcttggtaccgaattcgatatcctgcagagcggccgctctagagggcccctcgagtacccatacgacgtcccagactacgcttgagtttaaaccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggcttctgaggcggaaagaaccagcagatcgatctctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatgggtctgcattaatgaatcggccaaggatccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatcccttataaatcaaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggatcgaattaattcttaattaacatcatcaataatatacctt","created_at":"2024-08-28 20:21:39","updated_at":"2024-08-28 20:21:39","map":"\/vds\/map\/cat\/142","viewer":"\/vds\/viewer\/cat\/142","pivot":{"product_id":438,"map_id":142,"product_type":"App\\Models\\CatalogBaseMolecular"}}],"gene":null}