Anti-Cas9 Antibody
Cat. No.
|
Y300079
Print
|
Name
|
Anti-Cas9 Antibody
|
Unit
|
100 μl
|
Category
|
Monoclonal Antibodies
|
Application
|
WB,ELISA
|
Application Info
|
Working dilution must be determined by the user.
|
Raised In
|
Mouse
|
Clonality
|
Monoclonal
|
Reactivity
|
Other
|
Description
|
Anti-Cas9 Antibody is an antibody against Cas9, an essential RNA-guided DNA endonuclease observed originally in Streptococcus pyogene among other bacteria and archaea and a component of an adaptive microbial immune system that provides acquired immunity against foreign viruses and plasmids. Cas9 endonuclease is used to cleave double-stranded DNA directed by a short engineered guide-RNA (gRNA), leading to site-directed double strand breaks (DSB) in DNA submstrate. Thus, the CRISPR Cas9 system (clustered regularly interspaced short palindromic repeat CRISPR-associated protein 9) is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement.
Predicted size of 160kDa.
|
Immunogen
|
Recombinant fragment corresponding to Cas9 endonuclease from Streptococcus pyogenes.
|
Isotype
|
IgG1
|
Concentration
|
100 μg / 100 μl
|
Recommended Dilutions
|
Working dilution must be determined by the user.
|
Storage Buffer
|
PBS, pH 7.4 with 0.02% sodium azide.
|
Storage Condition
|
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
|
Formulation
|
Liquid
|
Species Reactivity
|
Other
|
Purity
|
Affinity purified from ascites fluid.
|
Guarantee
|
abm guarantees that all our Anti-Cas9 Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
|
Material Citation
|
If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Y300079
|
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