Anti-GFP Tag Antibody (Mouse Monoclonal)

Cat. No.

G096

Unit

100 μg

Price

$180.00

Cat. No.	G096
Name	Anti-GFP Tag Antibody (Mouse Monoclonal)
Unit	100 μg
Clone	LGB-1
Category	Tag Antibodies
Application	WB,ELISA,Other
Raised In	Mouse
Clonality	Monoclonal
Reactivity	Other
Description	Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.
Immunogen	GFP from the jellyfish Aequorea victoria N-Terminal peptide-KLH conjugates (first 20 aa).
Isotype	IgG1
Concentration	100ug/100ul
Recommended Dilutions	WB 1:1000. For other applications, the researcher should determine the optimal working dilution.
Storage Buffer	PBS, pH 7.4 with 0.05% sodium azide.
Storage Condition	This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Formulation	Liquid
Specificity	Recognizes native and denatured forms of GFP and its variants EGFP, YFP, EYFP, and CFP.
Purity	Affinity purified
Guarantee	abm guarantees that all our Anti-GFP Tag Antibody (Mouse Monoclonal) will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G096

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G096 Immunofluorescence QC

	Have the antibody been tested for Immunoprecipitation?
	Yes, and it works fine.

Moncivais, K et al. "Tetracycline repressor-based mammalian two-hybrid systems" Methods Mol Biol 812:259-273 (2012). DOI: 10.1007/978-1-61779-455-1_15. PubMed: 22218865. Application: Western Blot.
Kim, K et al. "Suppression of OsKu80 results in defects in developmental growth and increased telomere length in rice (Oryza sativa L.)" Biochem Biophys Res Commun 4:857-862 (2015). PubMed: 26590017.
Feng, Y., Hu, Y., Hou, Z., Sun, Q., Jia, Y., & Zhao, R. "Chronic corticosterone exposure induces liver inflammation and fibrosis in association with m 6 A-linked post-transcriptional suppression of heat shock proteins in chicken" Cell Stress and Chaperones 1-10: (2019).
Segal, I., Nachmias, D., Arbely, E., & Elia, N. "A straightforward approach for bioorthogonal labeling of proteins and organelles in live mammalian cells, using a short peptide tag" : (2019). DOI: 10.1101/708545.

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